Effect of the Monocarboxylate Transporter Inhibitor α-cyano-4-hydroxy-cinnamate on In Vivo Hyperpolarized MR Spectroscopic Imaging with [1-C]Pyruvate

Table 1: Quantitation over all tumor (for MYC) and liver (for healthy) voxels for mice in which MCT inhibition experiments were performed. Note: the pyruvate category includes pyruvate-hydrate. As shown by the data above, in all cases, alanine and lactate decreased after 4-CIN administration. Introduction: Development of hyperpolarized technology utilizing dynamic nuclear polarization has enabled the measurement of C metabolism in vivo at very high SNR [1]. Thus far, the most researched agent for in vivo applications has been [1-C]pyruvate. In this work, the role of cell membrane transport on the conversion of [1-C]pyruvate to [1-C]lactate and to [1-C]alanine in vivo was investigated by utilizing the monocarboxylate transporter (MCT) inhibitor α-cyano-4-hydroxy-cinnamate [2]. Reduced hyperpolarized alanine and lactate were detected after α-cyano-4hydroxy-cinnamate administration, which is in agreement with recent in vitro work where decreased hyperpolarized lactate was detected after breast cancer cells were given an MCT inhibitor [3].